Anti-inflammatory activity of Arnica montana
Keywords:
arnica montana, anti-inflamatory agents, plant extracts, medicinal plantsAbstract
Due to the almost non-existence of studies regarding Arnica at the systemic level, this study aims to prove the anti-inflammatory activity of Arnica montana. The Arnica montana solution used in this experiment was obtained from Arnica tincture. 88 Wistar rats were used; adults (150-200g), and were divided into three groups receiving, orally, a volume of 0.2ml/100g, for the control group:
distilled water with Tween, used to dilute the Arnica solution; in the experimental group: Arnica montana solution (10mg/ml) at a dose of 20mg/kg; in the positive control group: corticosteroid (Betamethazone) at a dose of 1mg/kg. After 60 minutes of treatment, the displacement of the mercury column was measured, caused by immersing the rat's right hind paw up to the lateral malleolus. The formation of edema, caused by the administration of 0.1 ml of formaldehyde, was also evaluated by displacement of the column of
mercury, at times: 30, 60, 120, 180 and 240 minutes after injection of the phlogistic agent. The results were expressed as differences in volume displaced between time zero and subsequent times. Arnica montana showed anti-inflammatory activity when it caused a reduction in the edena of the rat's paw caused by formaldehyde in relation to the control group of around 49.2; 40.6; 37.5; 37.9 and 33.7% at 30, 60, 120, 180 and 240 minutes respectively after edema induction. This action was less than that of the corticosteroid in the first hour (73%) and practically the same in the subsequent hours (91, 97 and 90%). It was also possible to observe that the animals showed similar behavior to the control group, not demonstrating toxic effects of Arnica at the dose used.
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References
ACCORSI, w.R., BARROS, M.A.A., ROCHELLE, L.A. Apontamentos sobre plantas tóxicas e medicinais. Piracicaba, 1982. p.96. Promovido pelo Departamento de Botânica/ESALQ/USP.
ARNICA montana dans les traumat de Ia face. Anuário Oto-Laryng, Paris, n. 1-2, p.94. 1976. (Seance Du).
BUSSE, W.W., KOPP, D.E., MIDDLETON JR, E. Flavonoid modulation of human neurotrophil function. J Allergy Clin Inununol, St. Louis, v.73, p.801-809 1984.
CAIRO, N. Guia de nteclicina honwopatica. 21.ed. São Paulo : Livraria Teixeira, 1988. p. 168-172.
COCHET, C. et al. Selective inhibition of a cyclic nucleotide independent protein kinase (G type casein kinase) by quercetin and related polyphenols. Biochem Pharmacol, Oxford, v.31, p. 1357-1361, 1982.
COSTA, A.F. Fannacognosia. 5.ed. Lisboa: Fundação Calouste Gulbnkian, 1994. v. 1, p.752-755.
CRAKER, L., SIMON, E., LANES E. Herbs, vices, and medicinal plants: recent advances in botany, horticulture, and pharmacology. New York: Orix Press, 1988. v.3, p. 103-144.
DAVIS, F.B., MIDDLETON JR., E., DAVIS, P.J., BLAS, S.D. Inhibition by quercetin of thyroid hormone stimulation in vitro of human red blood cell Ca2+- ATPase activity. Cell Calciunt, Edinburg, v.4, p.71-81, 1983.
DOMINGO NETO, A.L. Efeitos cicatrizantes e antimicrobianos das plantas medicinais. Piracicaba: UNICAMP, 1991. p.31, 80. Dissertação (Mestrado em Odontologia) -Unicamp, 1991.
GOODMAN, L.s., GILMAM, A.G. As bases farmacológicas da terapêutica. São Paulo McGraw-Hill do Brasil, 1997. p. 1086-1099.
GRYGLEWSKI, R.J., ROBAK, J., SWIES, J. Flavonoids lipoxygenases: platelet aggregation. NATO Advanced Studies Institutes. v.95, p. 149-166, 1985. (Serie A).
HACKETT, A.M. Plant flavonoids in biology and medicine: biochemical, pharmacological, and structure-activity relationships. New York : Alan R. Liss 1986. p. 177-194.
KATO, R. et al. Inhibition of 2-0 etradecanoylphorbol-13-acetate-induced tumor promotion and rnithine decarboxylase activityby quercetin: possible involvement of lipoxygenase inhibition. Carcinogenesis, New York, v.4y p. 1301-1305, 1983.
KATZUNG-BERTTRAN, G. Fannacologia båsica e clinica. 5.ed. Rio de Janeiro : Guanabara Koogan, 1994. p. 198-208.
KAZIRO, G.S.N. Metromidazolie (flagyl) and arnica montana is the prevention of post-sugical complications a comparative placebo controlled clinical trial. Br J Oral Maxil-Fa Surg, Edinburg, v.22, p.42-49, 1984.
LANNI, C., BECKER, E.L. Inhibition of neutrophil phospholipase A2 by p-bromophenylacyl bromide, nordihydroguaiaretic acid, eicosatetrayenoic acid and quercetin. Inter Arch Allergy Appl Inununal, Basel, v. 76, p.214-217, 1985.
LEE, T.P., MATTELIANO, M.L., MIDDLETON JR. E. Effect Of quercetin on human polymorphonuclear leukocyte lysosomal enzyme release and phospholipid metabolism. Life Sci, Oxford, v.20, p.2765-2774, 1982.
MORGAN, R. Enciclopédia das ervas e plantas medicinais. Säo Paulo : Hemus, 1995. p.47.
PELEGATTI, I. et al. Novo método para ensaio da atividade de drogas com agäo anti-inflamat6ria. Rev Fann Bioquim USP, São Paulo, v.8, n.2, p. 177-182, 1970.
SCHRODER, H. et al. Helenalin and 11x, 13-Didrohelenalin, two constituents from Arnica Montana L., Inhibit Haman Platelet Function Via Thiol: dependent path ways. Throntbosis Research, Elmsford, v.57, p.838-845, 1990.
WELTON, A.F. et al. Effect of flavonoids on arachidonic acid metabolism. In: CODY, V.E., MIDDLETON JR., E., HARBORNE, J.B. (Eds.). Plant flavonoids in biology and ntedicine: biochemical, pharmacological, and structure activity relationships, New York : Alan R. Liss, 1986. p. 231-242.
ZANINI, O. Guia de medicamento. Säo Paulo Atheneu, 1995. p. 104-108.
