The preventive effect of taxifolin on acrylamide-induced heart damage in rats
Keywords:
Acrylamide, Oxidative heart damage, Rat, TaxofilinAbstract
Objective
Acrylamide is a toxic compound widely used in industrial sectors. Acrylamide causes reactive oxygen species formation and the subsequent lipid peroxidation reaction, which plays an important role in the pathogenesis of oxidative damage. Taxifolin is a flavonoid with antioxidant properties that inhibit reactive oxygen species formation. In this study, we aimed to investigate the preventive effect of taxifolin on acrylamide-induced oxidative heart damage.
Methods
The rats were divided into three groups: Acrylamide, Acrylamide+Taxifolin , and Healthy group. Water and food intake and body weight alterations were recorded daily. Malondialdehyde, total glutathione, nuclear factor kappa-B, total oxidant status, and total antioxidant status levels were analyzed from the heart tissue. Troponin-I levels, the parameter known as a cardiac biomarker, were analyzed from the blood sample. The cardiac histopathologic examination was also performed.
Results
In the Acrylamide group animals, the malondialdehyde, nuclear factor kappa-B, total oxidant status, and troponin-I levels were significantly higher compared to the ones of Acrylamide+Taxifolin and Healthy groups. The levels of total glutathione and total antioxidant status were significantly lower compared to Acrylamide+Taxifolin and Healthy groups’. Additionally, in the Acrylamide group, body weight gain, food and water intake, significantly declined compared to the Acrylamide+Taxifolin and Healthy groups. However, in the Acrylamide+Taxifolin group, taxifolin supplementation brought these values close to Healthy group ones. Furthermore, taxifolin treatment ameliorated structural myocardial damage signs induced by acrylamide.
Conclusion
Acrylamide exposure significantly induced oxidative damage to rat heart tissue. Taxifolin was able to improve the toxic consequences of acrylamide biochemically and histopathologically, possibly due to its antioxidant properties.
References
Mousavi Khaneghah A, Fakhri Y, Nematollahi A, Seilani F, Vasseghian Y. The concentration of acrylamide in different food products: a global systematic review, meta-analysis, and meta-regression. Food Rev Int. 2020:1-19. https://doi. org/10.1080/87559129.2020.1791175
Maan AA, Anjum MA, Khan MKI, Nazir A, Saeed F, Afzaal M, et al. Acrylamide formation and different mitigation strategies during food processing: a review. Food Rev Int 2020:1-18. https://doi.org/10.1080/87559129.2020.1719505
Iriondo-DeHond A, Elizondo AS, Iriondo-DeHond M, Ríos MB, Mufari R, Mendiola JA, et al. Assessment of healthy and harmful maillard reaction products in a novel coffee cascara beverage: melanoidins and acrylamide. Foods. 2020;9(5):620. https://doi.org/10.3390/foods9050620
Yoosefian M, Pakpour A, Zahedi M. Carboxylated single-walled carbon nanotubes as a semiconductor for adsorption of acrylamide in mainstream cigarette smoke. Physica E. 2020;124:114299. https://doi.org/10.1016/j.physe.2020.114299
Pelucchi C, Rosato V, Bracci P, L-D, Neale R, Lucenteforte E, et al. Dietary acrylamide and the risk of pancreatic cancer in the International Pancreatic Cancer Case: Control Consortium (PanC4). Ann Oncol. 2017;28(2):408-14. https://doi.org/10.1093/annonc/mdw618
Guo J, Cao X, Hu X, Li S, Wang J. The anti-apoptotic, antioxidant and anti-inflammatory effects of curcumin on acrylamide-induced neurotoxicity in rats. BMC Pharmacol Toxicol. 2020;21(1):1-10. https://doi.org/10.1186/s40360-020-00440-3
Atabati H, Abouhamzeh B, Abdollahifar M-A, Javadinia SS, GharibianBajestanie S, Atamaleki A, et al. The association between high oral intake of acrylamide and risk of breast cancer: an updated systematic review and meta-analysis. Trends Food Sci Tech. 2020;100:155-63. https://doi.org/10.1016/j.tifs.2020.04.006
Sadat Yousefsani B, Akbarizadeh N, Pourahmad J. The antioxidant and neuroprotective effects of Zolpidem on acrylamide-induced neurotoxicity using Wistar rat primary neuronal cortical culture. Toxicol Rep. 2020;7:233-40. https://doi.org/10.1016/j.toxrep.2020.01.010
Huang M, Jiao J, Wang J, Xia Z, Zhang Y. Characterization of acrylamide-induced oxidative stress and cardiovascular toxicity in zebrafish embryos. J Hazard Mater. 2018;347:451-60. https://doi.org/10.1016/j.jhazmat.2018.01.016
Foroutanfar A, Mehri S, Kamyar M, Tandisehpanah Z, Hosseinzadeh H. Protective effect of punicalagin, the main polyphenol compound of pomegranate, against acrylamide-induced neurotoxicity and hepatotoxicity in rats. Phytother Res. 2020;34(12):3262-72. https://doi.org/10.1002/ptr.6774
Oppedisano F, Macrì R, Gliozzi M, Musolino V, Carresi C, Maiuolo J, et al. The anti-inflammatory and antioxidant properties of n-3 PUFAs: their role in cardiovascular protection. Biomedicines. 2020;8(9):306. https://doi.org/10.3390/biomedicines8090306
Kalinina I, Potoroko I, Sonawane SH. Sonochemical encapsulation of taxifolin into cyclodextrine for improving its bioavailability and bioactivity for food. In Sonawane SH, Bhanvase BA, Sivakumar M, editors. Encapsulation of active molecules and their delivery system. New York: Elsevier; 2020. p. 85-102.
Bedir F, Kocatürk H, Yapanoğlu T, Gürsul C, Arslan R, Mammadov R, et al. Protective effect of taxifolin against prooxidant and proinflammatory kidney damage associated with acrylamide in rats. Biomed Pharmacother. 2021;139:111660. https://doi.org/10.1016/j.biopha.2021.111660
Salaritabar A, Darvishi B, Hadjiakhoondi F, Manayi A, Sureda A, Nabavi SF, et al. Therapeutic potential of flavonoids in inflammatory bowel disease: a comprehensive review. World J Gastroenterol. 2017;23(28):5097-5114. https://doi.org/10.3748/wjg.v23.i28.5097
Ahiskali I, Pinar CL, Kiki M, Cankaya M, Kunak CS, Altuner D. Effect of taxifolin on methanol-induced oxidative and inflammatory optic nerve damage in rats. Cutan Ocul Toxicol. 2019;38(4):384-89. https://doi.org/10.1080/15569527.2019.1637348
Chen X, Gu N, Xue C, Li BR. Plant flavonoid taxifolin inhibits the growth, migration and invasion of human osteosarcoma cells. Mol Med Rep. 2018;17(2):3239-45. https://doi.org/10.3892/mmr.2017.8271
Terekhov RP, Selivanova IA, Tyukavkina NA, Ilyasov IR, Zhevlakova AK, Dzuban AV, et al. Assembling the puzzle of taxifolin polymorphism. Molecules. 2020;25(22):5437. https://doi.org/10.3390/molecules25225437
Alpan AL, Kızıldağ A, Özdede M, Karakan NC, Özmen Ö. The effects of taxifolin on alveolar bone in experimental periodontitis in rats. Arch Oral Biol. 2020;117:104823. https://doi.org/10.1016/j.archoralbio.2020.104823
Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979;95(2):351-8. https://doi.org/10.1016/0003-2697(79)90738-3
Sedlak J, Lindsay RH. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent. Anal Biochem. 1968;25:192-205. https://doi.org/10.1016/0003-2697(68)90092-4
Erel O. A new automated colorimetric method for measuring total oxidant status. Clin biochem. 2005;38(12):1103-11. https://doi.org/10.1016/j.clinbiochem.2005.08.008
Erel O. A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem. 2004;37(2):112-9. https://doi.org/10.1016/j.clinbiochem.2003.11.015
Ibrahim MA, Ibrahem MD. Acrylamide-induced hematotoxicity, oxidative stress, and DNA damage in liver, kidney, and brain of catfish (Clarias gariepinus). Environ Toxicol. 2020;35(2):300-8. https://doi.org/10.1002/tox.22863
Abdel-Daim MM, Abo El-Ela FI, Alshahrani FK, Bin-Jumah M, Al-Zharani M, Almutairi B, et al. Protective effects of thymoquinone against acrylamide-induced liver, kidney and brain oxidative damage in rats. Environ Sci Pollut Res Int. 2020;27(30):37709-17. https://doi.org/10.1007/s11356-020-09516-3
Mårtensson J, Jain A, Meister A. Glutathione is required for intestinal function. Proc Natl Acad Sci . 1990;87(5):1715-9. https://doi.org/10.1073/pnas.87.5.1715
Kushwah AS, KALIA TS. Quercetin attenuates oxidative stress, inflammation and cardiac dysfunction in acrylamideinduced cardiotoxicity. Acta Pol Pharm. 2020;77(2):343-52. https://doi.org/10.32383/appdr/110094
Yousef MI, El-Demerdash FM. Acrylamide-induced oxidative stress and biochemical perturbations in rats. Toxicology. 2006;219(1-3):133-41. https://doi.org/10.1016/j.tox.2005.11.008
Valentova M, Anker SD, von Haehling S. Cardiac cachexia revisited: the role of wasting in heart failure. Heart Fail Clin. 2020;16(1):61-9. https://doi.org/10.1016/j.hfc.2019.08.006
Hou J, Hu M, Zhang L, Gao Y, Ma L, Yan X, et al. Dietary taxifolin potently protects against dextran sulfate sodium-induced colitis via NF-κB signaling, enhancing ıntestinal barrier and modulating gut microbiota. Front Immunol. 2020;11:3915. https://doi.org/10.3389/fimmu.2020.631809
Tang Z, Yang C, Zuo B, Zhang Y, Wu G, Wang Y, et al. Taxifolin protects rat against myocardial ischemia/reperfusion injury by modulating the mitochondrial apoptosis pathway. PeerJ. 2019;7:e6383. https://doi.org/10.7717/peerj.6383
Unver E, Tosun M, Olmez H, Kuzucu M, Cimen FK, Suleyman Z. The effect of taxifolin on cisplatin-induced pulmonary damage in rats: a biochemical and histopathological evaluation. Mediators Inflamm. 2019;2019:1-7. https://doi.org/10.1155/2019/3740867
Li X, Xie H, Jiang Q, Wei G, Lin L, Li C, et al. The mechanism of (+) taxifolin’s protective antioxidant effect for OH-treated bone marrow-derived mesenchymal stem cells. Cell Mol Biol Lett. 2017;22(1):1-11. https://doi.org/10.1186/s11658-017-0066-9
Marković J, Stošić M, Kojić D, Matavulj M. Effects of acrylamide on oxidant/antioxidant parameters and CYP2E1 expression in rat pancreatic endocrine cells. Acta Histochem. 2018;120(2):73-83. https://doi.org/10.1016/j.acthis.2017.12.001
Zakaria N, Khalil SR, Awad A, Khairy GM. Quercetin reverses altered energy metabolism in the heart of rats receiving adriamycin chemotherapy. Cardiovasc Toxicol. 2018;18(2):109-19. https://doi.org/10.1007/s12012-017-9420-4
Mair J, Lindahl B, Hammarsten O, Müller C, Giannitsis E, Huber K, et al. How is cardiac troponin released from injured myocardium? Eur Heart J Acute Cardiovasc Care. 2018;7(6):553-60. https://doi.org/10.1177/2048872617748553
Sarkisian L, Saaby L, Poulsen TS, Gerke O, Jangaard N, Hosbond S, et al. Clinical characteristics and outcomes of patients with myocardial infarction, myocardial injury, and nonelevated troponins. Am J Med. 2016;129(4):e5-e21. https://doi.org/10.1016/j.amjmed.2015.11.006