Comparative study of mineral trioxide aggregate implanted in rat tibial bone defect
Keywords:
Biocompatible material, Bone implant, MTA, RatsAbstract
Objective
The objective of this study is to use morphometry to compare rat bone tissue response to two types of mineral trioxide aggregate: gray and white.
Methods
Mineral trioxide aggregate was implanted in tibial bone defects of 16 adult, male Wistar rats. Gray mineral trioxide aggregate was implanted in the left hind leg and white in the right hind leg. Another 16 animals were used as controls. Tissue samples
were collected for morphologic and morphometric analyses 2, 4, 6, 8 and 16 weeksafter the implant. The histological slides were stained with hematoxylin and eosin and analyzed with the software TPS Dig 1.38. The results were analyzed statistically using ANOVA followed by the Tukey test (p<0.05).
Results
The mineral trioxide aggregate was evidenced only in the periosteal region and adjacent soft connective tissue. Mineral trioxide aggregate was not found in the bone matrix.
Osteoblast proliferation and formation of primary bone in the fracture region occurred in the same patterns and proportions as those of the control animals. No type of cell infiltrate was observed in the periosteal region that could indicate an inflammatory process. Abnormal deposition of collagen fibers or significant amounts of newly formed vessels was also not observed. Morphometrically, there were no significant differences between the two types of mineral trioxide aggregate.
Conclusion
The two types of mineral trioxide aggregate did not interfere significantly with the bone regeneration process, thus they were compatible with the bone tissues in the experimental conditions used.
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References
Torabinejad M, Watson TF, Pittford TR. Sealing ability of a mineral trioxide aggregate when used as a root-end filling material. J Endod. 1993; 19(12):591-5.
Torabinejad M, Hong CU, Pittford TR, Kariyawasam SP. Tissue reaction to implated super-EBA and mineral trioxide aggregate in the mandible of guinea pigs: a preliminary report. J Endod. 1995a; 21(1 1):569-71.
Torabinejad M, Hong CU, Pittford TR, Kettering JD Citotoxicity of four root-end filling materiaIs. J Endod 1995b; 21(10),489-92.
Yatsushiro JD, Baumgartner JC, Tinkle JS. Longitudinal study of the microleakage of two root-end filling materiais using a fluid conductive system. J Endod. 1998; 24(11):716-9.
Osorio RM, Hefti A, Vertucci FJ, Shawley AL. Citotoxicity of endodontic materiais. J Endod. 1998; 24(2):91-6.
Oynick J. Oynick T. A study of a new material for retrograde fillings. J Endod. 1978; 4(7):203-6.
Smee G, Bolanos OR, Morse DR, Furst ML, Yesilsoy CA. A comparative leakage study of P-30 resin bonded ceramic, teflon, amalgam, and IRM as retrofilling seals. J Endod. 1987; 13(3):117-21.
Dorn SO, Gartner AH. Retrograde filling materiais: a retrospective success-failure study of amalgam, EBA e IRM. J Endod. 1990; 16(8):391-3.
Regan JD, Gutman JL, Witherspoon DE, Comparison of Diaket and MTA when used as root end filling materiaIs to support regeneration of the perirradicular tissues. Int Endod J. 2002; 35(10):840-7.
Shabahang S, Torabinejad M, Boyne PP, Abedi H, McMillin P A comparative study of root end inducüon using osteogenic protein-1, calcium hydroxide, and mineral trioxide aggregate in dogs. J Endod. 1999; 25(1):1 -5.
Economides N. Pantelidou O. Kokkas A. Tziafas D Short-term perirradicular tissue response to mineral trioxide aggregate (MTA) as root end filling material, Int Endod J. 2003; 36(1):44-8.
Ketser K, Johnson C, Tipton DA, Cytotoxicity of mineral trioxide aggregate using human periodontal ligament fibroblasts. J Endod. 2000; 26(5):288-91.
Lee SJ, Monsef M, Torabinejad M, Sealing ability of a mineral trioxide aggregate for repair of lateral root perforations. J Endod. 1993; 19(11):541-4.
Torabinejad M, Rastegar AF, Kettering JD, Pittford TR. Bacterial leakage of Mineral Troxide Aggregate as a root-end filling material. J Endod. 1995c; 21 (3):109-12.
Torabinejad M, Hong UJ, McDonald F, Pittford TR, Physical and chemical properties of a new root end filling material. J Endod. 1995d; 21 (7):349-53.
Torabinejad M, Smith PW, Kettering JD, Pittford TR. Comparative investigation of marginal adaptation of mineral trioxide aggregate and other commonly used root-end filling materiaIs. J Endod. 1995e; 21 (6):295-9.
Koh ET, Torabinejad M, Pittford TR, McDonald f. Mineral trioxide aggregate stimulates a biological response in human osteoblasts. J. Biomed Mater Res 1997; 37(3):432-9.
Koh ET, McDonald F, Pittford TR, Torabinejad M Cellular response to mineral trioxide aggregate. J Endod. 1998; 24(8):543-7.
Asrari M. Lobner D. In vitro neurotoxic evaluation of root-end-filling materiaIs. J Endod. 2003; 29(1 1 ):743-6.
Holland R, Souza V, Nery MJ, Otoboni Filho JA, Barnabé PFE. Dezan Júnior E, Reaction of rat connective tissue to implanted dentin tubes with mineral trioxide aggregate or calcium hydroxide. J Endod. 1999; 25(3): 161-6
Torabinejad M, Hong CU. Lee SJ. Monsef M, Pittford TR, Investigation of mineral trioxide aggregate for root-end filling in dogs. J Endod 1 995b; 21(12),603-821.
Torabinejad M, Pittford TR, McKendry DJ, Abedi HR, Miller DA, Kariyawasam SP. Histologic assessment of mineral trioxide aggregate as a rot-end filling in monkeys. J Endod. 1997; 23(4):225-8.
MTA: instruções de uso - Angelus, Londrina,[acesso em 31 out. 2008]. Disponível em: <http://www.angelus.ind.br/arquivos/fileconteudo/290_file. pdf/>
Faraco IM, Holland R. Histomorpholgical response of dogs’dental pulp capped with white mineral trioxide aggregate. Braz Dent J. 2004; 15(2):104-8.
Schwartz RS, Mauger M, Clement DJ, Walker III WA Trióxido mineral agregado: um novo material para Endodtia. JADA. 1999; 2(5):44-52.
Camilleri J, Montesin FE, Papaioanou S, McDonald F, Pittford TR. Biocompatibility of two commercial forms of mineral trioxide aggregate. Int Endod J. 2004; 37(10):699-704.
Shahi S, Rahimi S, Lotfi M, Yavari H. Gaderian A. A comparative study of the biocompatibility of three root-end filling materials in rat connective tissue. J Endod. 2006; 32(8):776-80.
Noetzel J, C)zer K, Reisshauer BH. Anil A. Rossler R, Neumann K, et al. Tissue responses to an experimental calcium phosphate cement and mineral trioxide aggregate as materials for furcation perforation repair: a histological study in dogs. Clin Oral Investig 2006; 10(1):77-83.
Morais CA. Bernardineli N. Garcia RB. Duarte MA. Guerisoli DM. Evaluation of tissue response to MTA and Portland cement with iodoform, Oral Surg Oral Med Oral Pathol Oral Radiof Endod. 2006: 102(3):417-21.
