Physical, chemical and biomechanical bone response of female ovariectomized rats to various intakes of supplemental fluorine
Keywords:
biomechanics, fluorine, menopause, osteoporosis, ovariectomyAbstract
Objective
This study investigated the effects of supplemental fluorine on the bone characteristics of ovariectomized (castrated) female rats, used as a model for experimental osteoporosis, evaluating the correlation between biomechanical parameters and the physical and chemical bone properties.
Methods
Seventy-eight female, 4-month-old Wistar rats were divided into 8 groups: the basal group, generating the initial values for the parameters; group 1, consisting of non-ovariectomized rats; and groups 2 to 7, consisting of ovariectomized rats that ingested increasing concentrations of supplemental sodium fluoride solutions (NaF): 0, 20, 40, 60, 80 and 100mg/L, respectively, for two months.
Results
Ovariectomy promoted osteopenia, determined from the morphometric, physical and chemical parameters and causing lower biomechanical resistance of the femurs submitted to flexion trials. The bones of the rats that ingested the 40mg/L fluorine solution demonstrated better physical, biochemical and biomechanical conditions than those of the non-treated ovariectomized and intact control rats.
Conclusion
Besides promoting protection against the bone loss induced by estrogen deficiency, fluorine improved bone quality, which was demonstrated by the enhanced biomechanical results as compared to the intact group
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