Neonatal malnutrition and normal microbiota of the oral cavity in rats
Keywords:
bacteria, aerobic, mouth, neonatal malnutrition, rats WistarAbstract
Objective
To evaluate the influence of neonatal malnutrition on the pattern and growth of aerobic bacteria of the normal bacterial flora of the oral cavity in adults Wistar rats.
Methods
In the present study, the material of the oral cavity was collected through swabs soaked in 40µL of sterile saline solution. After the collection, each swab was placed in a sterile tube containing 960µL of brain heart infusion. Later, the samples were homogenized. Then, from the 1.000µL, 1µL was collected with a gauged loop to be sowed in Petri dishes containing Agar-blood and Agar-Levine, for the isolation and identification of the Grampositive and Gram-negative bacteria respectively. The plates were placed into a bacteriological incubator, 37ºC, for 48 hours and the colony-forming units that grew were counted and their percentages were calculated. For bacterioscopy, slides were stained with the Gram method.
Results
From the 5th to the 21st day of life, body weight of the undernourished group (33.6g:42.8g, standard deviation=27.2g) was smaller (p<0.001) than that of the nourished group (52.5g:56.3g, standard deviation=24.0g). From the 60th to the 120th days of life the body weight of the undernourished group (7.4.x.106 colony-forming units, standard deviation=1,0x106 colony-forming units bacteria/mL) decreased when compared to that of the nourished group (306.2g:319.4g, standard deviation=219.5g), Mann-Whitney test, p<0,05. The microbiota was composed of a smaller number of bacteria in the nourished group (4.2 x106 colony-forming units, standard deviation=1.2x106 colony-forming units bacteria/mL) when compared with the undernourished group (7.4x106 colony-forming units, standard deviation=1.0x106 colony-forming units bacteria/mL), Student’s “t” test (p=0.026). From the bacteria isolated in cultures, a similar bacterial pattern was observed in both groups, with 70% of Gram-positive bacteria and 30% of Gram-negative bacteria.
Conclusion
Neonatal malnutrition was effective in decreasing body weight and increasing bacterial growth. Although the bacterial pattern was not altered, the increase in bacterial growth may induce an imbalance of the oral microbiota, being a threat to the host.
References
Mandell GL, Douglas RG, Bennett JE, Dolin R. Principles and practice of infectious diseases. 5th ed. Philadelphia: Churchill Livingstone; 2005.
Paster BJ, Boches SK, Galvin JL, Ericson RE, Lau CN, Levanos VA, et al. Bacterial diversity in human subgingival plaque. J Bacteriol. 2001; 183(1): 3770-83.
Burton GRW, Engelkirk PG. Microbiologia: para as ciências da saúde. Rio de Janeiro: Guanabara Koogan; 2005.
Lindhe, J. Microbiologia da doença periodontal associada à placa. In: Tratado de periodontologia clínica. 2a. ed. Rio de Janeiro: Guanabara; 1992.
Gutierrez-Perez JL, Perea-Perez EJ, Romero-Ruiz MM, Giron-Gonzales JA. Orofacial infections of odontogenic origin. Méd Oral. 2004; 9(1):280-7.
Tortora GJ, Funke BR, Case CL. Princípios de doença e epidemiologia. In: Microbiologia. 6a. ed. Porto Alegre: Artes Médicas Sul; 2000. p.394-420.
Trabulsi LR. Flora normal do corpo humano. In: Microbiologia. Rio de Janeiro: Atheneu; 1986. p.35-7.
Isogai E, Isogai H, Sawada H, Kaneko H, Ito N. Microbial ecology of plaque in rats with naturally occurring gingivitis. Infect Immun. 1985; 489(1): 520-7.
Commitee on Laboratory Animal Diets. Assembly of life sciences. National Research Council. Control of diet in laboratory animal experimentation. Nutr Abstr Rev. 1979; 40(1):413-9.
Medeiros JMB, Feitoza EG, Magalhães. Lima K, Cabral Filho JE, Castro FMM, et al. Malnutrition during brain growth spurt alters the effect of fluoxetine on agressive behavior in adult rats. Nutr Neurosci (USA). 2004; 7(1):49-52.
Porto S, Araujo FRG, Melo JF, Silva RR, Silva KMF, Castro CMMB. Cinética do perfil leucocitário do sangue, antes e após treinamento físico moderado, em ratos adultos desnutridos no período neonatal. Anais Fac Med Univ Fed Pernambuco. 2006; 51(2):59-65.
Barros KMFT, Manhães de Castro R, Sousa SL, Matos RJB, Deiró TCBJ, Cabral Filho JE, et al. A regional model (Northeast Brazil) of induced malnutrition delays ontogeny of reflexes and locomotor activity in rats. Nutr Neurosci (UK). 2006; 9(0):99-104.
Teodósio NR, Lago ES, Romani SAM, Guedes RCA. A regional basic diet from Northeast Brazil as a dietary model of experimental malnutrition. Plant Foods Hum Nutr. 1990; 40(4):289-96.
Pyne DB, Gleeson M. Effects of intensive training on immunity in athletes. Int J Sports Med. 1998; 19(1):138-94.
Brown JL, Pollitt E. Malnutrition, poverty and intellectual development. Sci Am. 1996; 2(1): 38-43.
Chandra RK. Nutrition and the immune system: an introduction. Am J Clin Nutr. 1997; 66(2): 460S-3S.
Totti MAG, Jorge AOC, Rego MA, Almeida DP. Recuperação de Candida Albicans, C. Tropicalis, C. Guilliermondii e C. Krusei na cavidade bucal de ratos normais e sialoadenectomizados. Rev Odontol UNESP. 1996; 25(1):119-24,
Koller MM, Maeda N, Nowzari H, Jorgensen MG. Desipramine changes salivary gland function, oral microbiota, and oral health in rats. Eur J Pharmacol. 2000; 408(1):91-8.
Darby I, Curtis M. Microbiology of periodontal disease in children and young adults. Periodontology 2000. 2001; 26(1):33-53.
Socransky SS, Haffajee AD. Periodontal microbial ecology. Periodontology 2000. 2005; 38(1): 135-87.
Marotta F, Barreto R, Kawakita S, Minelli E, Pavasuthipaisit K, Lorenzetti A, et al. Preventive strategy for Candida gut translocation during ischemia-reperfusion injury supervening on protein-calorie malnutrition. Chin J Dig Dis. 2006; 7(1):33-8.
Marcotte H, Lavoie MC. Oral microbial ecology and the role of salivary immunoglobulin A. Microbiol Mol Biol Rev. 1998; 62(1):71-109.
Maitland K, Berkley JA, Shebbe M, Peshu N, English M, Newton CR. Children with severe malnutrition: can those at highest risk of death be identified with the WHO protocol? PLoS Med. 2006; 3(12):e500.
Ye P, Shearer CK, Yates MG. Streptococci and Actinomyces induce antibodies which cross react with epithelial antigens in periodontitis. Clin Exp Immunol. 2003; 131(1):468-76.
Yao ES, Lamont RJ, Leu SP. Interbacterial binding among strains of pathogenic and commensal oral bacterial species. Oral Microbiol Immunol. 1996; 11(1):35-41.
Beckers HJ, van der Hoeven JS. The effects of mutual interaction and host diet on the growth rates of the bacteria Actinomyces viscosus and Streptococcus mutans during colonization of tooth surfaces in di-associated gnotobiotic rats. Arch Oral Biol. 1984; 29(3):231-6.
Hamada S, Slade HD. Biology, immunology and cariogenicity of Streptococcus mutans. Microbiol Rev. 1980; 44(2):331-84.
Trudel L, St-Amand L, Bareil M, Cardinal P, Lavoie MC. Bacteriology of the oral cavity of the BALB/c mice. Can J Microbiol. 1986; 32(1):673-8.
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Copyright (c) 2023 Solange Maria Magalhães da Silva PORTO, Marcelo Tavares VIANA, Karla Melo Ferreira da SILVA, Maria de Fátima Alves DINIZ, Célia Maria Machado Barbosa de CASTRO
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