Thirty-day β-hydroxy-β-methylbutyrate supplementation with a controlled diet does not alter the morphological and morphometric characteristics of the rat diaphragm muscle
Palavras-chave:
Diaphragm, Histology, Rats Wistar, Supplementary feedingResumo
Objective
To evaluate the b-hydroxy-b-methylbutyrate supplementation infl uence on the morphological and morphometric characteristics of the diaphragm muscle of rats and verify whether there are sex differences.
Methods
Experimental study with 48 Wistar rats (24 of each sex) divided into 3 groups: Control Group: in which a daily diet with saline solution was offered; Experimental Group: the same amount of food from the Control group consumed in the previous day and 0.3g/kg of β-hydroxy-β-methylbutyrate; Ad libitum Experimental Group: ad libitum feeding with the same dose of the supplement. The analysis consisted of histomorphometry and classification in diaphragm muscle fiber area bands. The procedures occurred 30 days after the start of the experiment. Data were analyzed using the one-way ANOVA and Tukey tests (p<0.05).
Results
There was no increase in the cross-sectional area of the diaphragm muscle fibers with the supplementation protocol employed and a similar histological pattern in both sexes. No significant changes were observed in muscle fiber size ranges in the supplemented groups, suggesting that there was no hypertrophy of muscle fibers.
Conclusion
This study suggests that β-hydroxy-β-methylbutyrate supplementation does not cause changes in the morphological and morphometric characteristics of the diaphragm muscle, regardless of sex.
Referências
Kennedy ET, Luo H, Houser RF. Dietary supplement use pattern of U.S. adult population in the 2007-2008 National Health and Nutrition Examination Survey (NHANES). Ecol Food Nutr. 2013;52(1):76-84.
Malafarina V, Uriz-Otano F, Iniesta R, Gil-Guerrero L. Effectiveness of nutritional supplementation on muscle mass in treatment of sarcopenia in old age: A systematic review. J Am Med Dir Assoc. 2013;14(1):10-7.
Li N, Hauser R, Holford T, Zhu Y, Zhang Y, Bassig BA, et al. Muscle-building supplement use and increased risk of testicular germ cell cancer in men from Connecticut and Massachusetts. Br J Cancer. 2015;112(7):1247-50.
Wilson JM, Fitschen PJ, Campbell B, Wilson GJW, Zanchi N, Taylor L, et al. International Society of Sports Nutrition Position Stand: beta-hydroxy-beta-methylbutyrate (HMB). J Int Soc Sports Nutr. 2013;10(6):1-14.
Wilkinson DJ, Hossain T, Hill DS, Phillips BE, Crossland H, Williams J, et al. Effects of leucine and its metabolite β-hydroxy-β-methylbutyrate on human skeletal muscle protein metabolism. J Physiol. 2013;591(11):2911-23.
Always SE, Pereira SL, Edens NK, Hao Y, Bennett BT. β-Hydroxy-β-methylbutyrate (HMB) enhances the proliferation of satellite cells in fast muscles of aged rats during recovery from disuse atrophy. Exp Gerontol. 2013;48(9):973-84.
Nissen SL, Sharp RL. Effect of dietary supplements on lean mass and strength gains with resistance exercise: A meta-analysis. J Appl Physiol. 2003;94(2):651-9.
Jówko E, Ostaszewski P, Jank M, Zieniewicz A, Wilszack J, Nissen S. Creatine and β-hydroxy β-methylbutyrate (HMB) additively increase lean body mass and strength during a weight training program. Nutrition. 2001;17(7-8):558-66.
Nissen S, Sharp RL, Panton L, Vukovich M, Trappe S, Fuller Junior JC. Beta-hydroxy-beta-methylbutyrate (HMB) supplementation in humans is safe and may decrease cardiovascular risk factors. J Nutr. 2000;130(8):1937-45.
Nissen S, Abumrad NN. Nutritional role of the leucine metabolite β-hydroxy- β-methylbutyrate (HMB). J Nutr Biochem. 1997;8:300-11.
Mirza KA, Pereira SL, Voss AC, Tisdale MJ. Comparison of the anticatabolic effects of leucine and Ca-β-hydroxy-β-methylbutyrate in experimental models of cancer cachexia. Nutrition. 2014;30(7-8):807-13.
Fitschen PJ, Wilson GJ, Wilson JM, Wilund KR. Efficacy of β-hydroxy-β-methylbutyrate supplementation in elderly and clinical populations. Nutrition. 2013;29(1):29-36.
Armstrong DG, Hanft JR, Driver VR, Smith APS, Lazaro-Martinez JL, Reyzelman AM. Effect of oral nutritional supplementation on wound healing in diabetic foot ulcers: A prospective randomized controlled trial. Diabet Med. 2014;31(9):1069-77.
Wu H, Xia Y, Jiang J, Du H, Guo X, Liu X, et al. Effect of beta-hydroxy-beta-methylbutyrate supplementation on muscle loss in older adults: A systematic review and meta-analysis. Arch Gerontol Geriatr. 2015;61(2):168-75.
Wilson JM, Lowery RP, Joy JM, Andersen JC, Wilson SMC, Stout JR, et al. The effects of 12 weeks of beta-hydroxy-beta-methylbutyrate free acid supplementation on muscle mass, strength, and power in resistance-trained individuals: A randomized, double-blind, placebo-controlled study. Eur J Appl Physiol. 2014;114(6):1217-27.
Supinski GS, Callahan LA. β-hydroxy-β-methylbutyrate (HMB) prevents sepsis-induced diaphragm dysfunction in mice. Respir Physiol Neurobiol. 2014;196(1):63-8.
Gebhardt A, Pancherz H. The effect of anabolic steroids on mandibular growth. Am J Orthod Dentofacial Orthop. 2003;123(4):435-40.
Hao Y, Jackson JR, Wang Y, Edens N, Pereira SL, Always SE. β-Hydroxy-β-methylbutyrate reduces myonuclear apoptosis during recovery from hind limb suspension-induced muscle fiber atrophy in aged rats. Am J Physiol Regul Integr Comp Physiol. 2011;301(3):701-15.
Krzysztof D, Jan J. The efficacy of a β-hydroxy-β-methylbutyrate supplementation on physical capacity, body composition and biochemical markers in elite rowers: A randomised, double-blind, placebo-controlled crossover study. J Int Soc Sports Nutr. 2015;12(31):1-11.
Daré LR, Dias DV, Rosa Junior GM, Bueno CRS, Buchaim RL, Rodrigues AC, et al. Effect of b-hydroxy-bmethylbutyrate in masticatory muscles of rats. J Anat. 2015;226(1):40-6.
Wheatley SM, El-Kadi SW, Suryawan A, Boutry C, Orellana RA, Nguyen HV. Protein synthesis in skeletal muscle of neonatal pigs is enhanced by administration of β-hydroxy-β-methylbutyrate. Am J Physiol Endocrinol Metab. 2014;306(1):e91-9.
Davis TA, Fiorotto ML, Burrin DG, Reeds PJ, Nguyen HV, Beckett PR, et al. Stimulation of protein synthesis by both insulin and amino acids is unique to skeletal muscle in neonatal pigs. Am J Physiol Endocrinol Metab. 2002;282(4):880-90.
Downloads
Publicado
Como Citar
Edição
Seção
Licença
Copyright (c) 2023 Cleuber Rodrigo de Souza BUENO, Bruno MARTINELLI, Gabriele Candido BERNARDO, Leticia Rossi DARÉ, Jesus Carlos ANDREO, Geraldo Marco ROSA JUNIOR
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
