Hematological and phagocytic response of the fat snook, Centropomus parallelus, reared in net cages, before and after inoculation with Sacharomyces ceresivisiae
Keywords:
Phagocytosis, Hematology, Macrophage migration, Fat snookAbstract
The aim of this study was to examine the blood and the macrophagocytic response of fat snook of Centropomus parallelus before and after injection with Saccharomyces cerevisiae. Ten adults were used, caught using net cages in the lagoon-estuarine region of Cananéia (SP), Brazil. The fi sh were anesthetized and blood was drawn followed by inoculation with yeast. After 8 hours, the fi sh were again removed from the cages and anesthetized. A new sample of blood was collected and the animals were then sacrifi ced by deep sedation. The blood was used for the determination: erythrocyte count, hemoglobin concentration and hematocrit, calculation of Mean Corpuscular Volume and Mean Corpuscular Hemoglobin Concentration, and preparation of smears, utilized for total and diff erential leukocyte counts white blood cells and thrombocyte count. There was a signifi cant decrease in almost all parameters 8hrs after inoculation (p0.05), with exception of mean corpuscular volume which showed a signifi cant increase (p>0.05). Leukopenia, lymphopenia, neutropenia and monocytosis occurred when comparing the values of the fi rst and second samples, in both inoculated and non inoculated fi sh. With the white blood cells, lymphocytes and neutrophils, values were signifi cantly higher in the inoculated fi sh versus those which were not inoculated, in both blood samplings. The number of thrombocytes was higher in inoculated than non inoculated individuals, but 8 hours after inoculation the values in the two groups were similar.
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Bennani, N.; Schimid-Alliana, A. & Lafaurie, M. (1995). Evaluation of phagocytic activity in a teleost fish, Dicentrarchus labrax. Fish and Shellfish Immunology, 5(2):237-46.
Blazer, B.S.; Wolke R.E.; Brown J. & Powell C.A. (1987). Piscine macrophage aggregate parameters as health monitors: Effect of age, sex, relative weight, season and site quality in largemouth bass (Micropterus salmoides). Aquatic Toxicology, 10(4):199-215.
Blazer, V.S. (1991). Piscine macrophage function and nutritional influences: A review. Journal of Aquatic Animal Health, 3(2):77-86.
Bodamer, J.E. & Robohm, R.A. (1996). Ultrastructural observation on the phagocytic behavior of winter flounder Pleuronectes americanus peritoneal neutrophils and macrophages in vivo. Disease of Aquatic Organisms, 25(3):197-208.
Bouck, G.R. & Ball, R.C. (1966). Influence of capture methods on blood characteristics and mortality in the rainbow trout (Salmo gairdneri). Transactions of the American Fisheries Society, 95(2):170-6.
Cerqueira, V.R. (2004). Cultivo de peixes marinhos. In: Poli, C.R.; Poli, A.T.B.; Andreatta, E.; Beltrane, & E. Aqüicultura: experiências brasileiras. Florianópolis: Universidade Federal de Santa Catarina. p.369-406.
Collazos, M.E.; Ortega, E.; Barriga, C. & Rodrigues, A.B. (1998). Seasonal variation in hematological parameters in male and female Tinca tinca. Molecular and Cellular Biochemistry, 183(1-2):165-8.
Hrube, T.C. & Smith, S.A. (1998). Hematology of fish. In: Feldman, B.F.; Zinkl, J.G. & Jain, N.C. (Ed.). Schalm’s Veterinary Hematology. 5th ed. Blackburg: Wiley- Blackwell. p.1120-5. Imagawa, T.; Hashimoto, Y.; Kitagawa, H.; Kon, Y.; Kudo,
Y.N. & Sugimura, M. (1989). Morphology of blood cells in Carp (Cyprinus carpio L.). Japanese Journal of Veterinary Science, 51(6):1163-72.
Iwama, G.K.; Greer, G.L. & Randall, D.J. (1986). Changes in selected haematological parameters in juvenile chinook salmon subjected to a bacterial challenge and a toxicant. Journal of Fish Biology, 28(5):563-72.
Kang, J.-C.; Kim, S.-G. & Jang, S.-W. (2005). Changes of Rock-fish, Sebastes schlegeli (Hilgendorf) exposed to dietary Cu and Cd. Journal of World Aquatic Society, 36(2):188-95.
Kodama, H.; Tijiwa, K.; Moritomo, T. & Nakanishi, T. (2002). Granulocyte response to experimental injection of live and formalin-killed bacteria in carp (Cyprinus carpio). Veterinary Immunology Immunopathology, 90(1-2):101-5.
Matushima, E.R. & Mariano, M. (1996). Kinetics of the inflammatory reaction induced by carrageenin in the swimbladder of Oreochromis niloticus (Nile-Tilapia). Brazilian Journal of Veterinary Research of Animal Science, 33(1):5-10.
Oliver, G.; Eaton, C.A. & Campbell, N. (1986). Interaction between Aeromonas salmonicida and peritoneal macrophages of brook trout (Salvelinus fontinalis). Veterinary Immunology Immunopathology, 12(1-4): 223-34.
Pulsford, A. (1984). Preliminary studies on trypanosomes from the dogfish, Scyliarhinus canicula L. Journal of Fish Biology, 24 (6):671-82.
Ranzani-Paiva, M.J.T. (1995a). Características hematológicas de tainha, Mugil platanus Günther, 1880 (Osteichthyes, Mugilidae) da região estuarino-lagunar de Cananéia -SP (Lat. 25° 00’S - Long. 47° 55’W). Boletim do Instituto de Pesca, 22(1):1-22.
Ranzani-Paiva, M.J.T. (1995b). Células sangüíneas e contagem diferencial dos leucócitos de tainha, Mugil platanus Günther, 1880 (Osteichthyes, Mugilidae) da região estuarino-lagunar de Cananéia - SP (Lat. 25° 00’S - Long. 47° 55’W). Boletim do Instituto de Pesca , 22 (1): 23-40.
Ranzani-Paiva, M.J.T. & Silva-Souza, A.T. (2004). Hematologia de peixes brasileiros. In: Ranzani-Paiva, M.J.T.; Takemoto, R.M.; & Lizama. M.A.P. Sanidade de organismos aquáticos. São Paulo: Varela. p.89-120.
Ranzani-Paiva, M.J.T.; Ishikawa, C.M.; Eiras, A.C. & Silveira, V.R. (2004). Effects of an experimental challenge with
Mycobacterium marinum on the blood parameters of Nile tilapia, Oreochromis niloticus (Linnaeus, 1757). Brazilian Archives of Biology and Technology, 47(6): 945-53.
Rosenfeld, G. (1947). Corante pancrômico para a hematologia e citologia clínica: nova combinação dos componentes do May-Grünwald e do Giemsa num só corante de emprego rápido. Memórias do Instituto Butantan, 20:329-334.
Rowley, A.F. & Ratcliffe, N.A. (1998). Vertebrate blood cells.
New York: Cambridge University Press.
Saad, M.A.H.; Ezzat, A. & Shabana, M.B. (1973). Effects of pollution on the blood characteristics of Tilapia zilli G. Water Air Soil Pollutant, 2(2):171-9.
Silva, J.R.M.C.; Hernandez-Blazquez, F.J.; Barbieri, R.L. (1998). Induced inflammatory response in the Antarctic fish. Notothenia Neglecta Polar Biology, 20(3):206-12.
Soivio, S.; Westmann, K. & Nyholm, K. (1974). The influence of change in oxygen tension on the haematocrit value of blood samples from asphyxic rainbow trout (Salmo gairdneri). Aquaculture, 3(4):395-401.
Veiga, M.L.; Egami, M.I.; Ranzani-Paiva, M.J.T. & Rodrigues, E.L. (2000). Aspectos morfológicos y citoquímicos de las células sanguíneas de Salminus maxillosus Valenciennes, 1840 (Characiformes, Characidae). Revista Chilena de Anatomia, 18(2):245-50.
Veiga, M.L.; Egami, M.; Ranzani-Paiva, M.J.T. & Rodrigues, E.L. (2002). Morphological and ultrastructural study of the thrombocytes and leukocyte granulocytes of Salminus maxillosus (Pisces, Characidae). Submicroscopic Cytology and Pathology, 34(4):397-402.
Wedemeyer, G. (1970). Stress of anesthesia with MS 222 and benzocaine in rainbow trout (Salmo gairdneri). Journal of Fisheries Research Board of Canada, 27(5): 909-14.
Zar, J. H. (1996). Biostatistical Analysis. 3rd ed. Upper Saddle River, New Jersey: Prentice-Hall.
Zelikoff, J.T.; Enami, N.A., Bowser, D.; Squibb, S. & Frenel, K. (1991). Development of fish peritoneal macrophages as a model of higher vertebrates in immunotoxicological studies. 1. Characterization of trout macrophages morphological, functional and biochemical properties. Fundamental Applied Toxicology, 16(3):576-89.
