Unidade móvel para cultivo de anaeróbicos mobile unit for anaerobic culture
Resumo
It has beco me clear in recent years that anaerobic bacteria are important causes of many different types of infection. Any type of bacterial infection in humans may involve anaerobes. Holding and transporting specimens containing anaerobes in transport media, saline, or culture media may often lead to loss of viabilityof obligate anaerobes and to the overgrowth of facultatively anaerobic organisms from mixed culture infections. The holding-transport procedure now recommended by most pattern anaerobic laboratories is to place ali.sp~cimens into steriJe, dry, oxygen-free, CO2-filled stoppered tubes and to hold it at room temperature. Alternatively, specimens should be cultured immediately. The present work describes an alternative equipment to be used as a mobile unit for anaerobicculture which may be moved into hospital room, operatory or wherever necessary to obtain clinical specimens for laboratory study utilizing the pre-reduced media and techniques recommended by the anaerobic pattern laboratories.
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Referências
FINEGOLD5,. M. (1977).Anaerobic bacteria in human disease, New York, Academic Press, Inc.
__________& MARTIN,W. J. (1982). Diagnostic Microbiology, 6 th Ed, 5t. Louis, The C. V. Moshey Co.
BUCHANAN,R.E.& GIBBON,N. E. (1974)editors: Bergey's Manualof Determinative Bacteriology ed. 8; Baltimore. The Williams and Wilkins Co.
FELNER, J. M. & DOWELL, V. R. Jr. "Bacteroides" bacteremia, Am. J. Med. 50: 787-796, 1971
GONZALES-C, C. L. & CALlA, F. M. (1975) Bacteriologic flora of aspiration induced pulmonary infections, Arch Intern. Med. 135: 711-714
BALOWS, A.; DEHAAN, R. M. ; DOWELL, V. R. Jr; editors. (1974). Anaerobic bacteria: role in disease, Springfield, 11., Charles C. Thomas, Publisher.
BEERENS,H. & TAHON-CASTEL, M. (1965) Infection humaines à Bactéries Anáerobies Non Toxigênes Bruxelles, Presses Académiques
BEAUCAGE, C. M. & ONDERDONK, A. B. (1982) Evaluation of pre-reduced anaerobically sterilized medi um (PRAS 11) System for indentification of anaerobic microrganisms, J. Clin.Microbiol. 16: 570-572
DOWELL, V. R. Jr., & HAWKINS, T. M. (1974) Laboratory methods in anaerobic bacteriology CDC Laboratory Manual, DHEW Publication No (CDC) 74-8272, Washington, D. V.S. Govermment Printing office. C"
__________& LOMBARD, G. L. (1981) Reactions of anaerobic bacteria in differential agar media, U. S. Department of Heath and Human Services, Public Heath Service, Center for Disease Control, Atlanta, GA.
__________& __________ THOMPSON, F. S.; ARMFIELD, A. Y. (1977). Media forisolation, characterization, and indetification of obligately anaerobic bacteria, Atlanta, GA, Center for Disease Control.
GOODMAN, A. D. (1977). Isolation of anaerobic bacteria from the root canal systems of necrotic teeth by use of a transport solution, Oral Sugar 43: 766-770.
MURRAY, P. R. & SONDAG, J. E. (1978) Evaluation ofroutine subcultures of macroscopically negative blood cultures for detection of anaerobes, J. Clin. Microbiol. 08: 427-430
National Comunitee for clinical laboratory Standards. (1978) Standard procedures for handling and transport of diagnostic medicar specimens and etiologic agents, TSH-5, NCCLS, Villanova, PA, the Comunitee.