Os efeitos em Allium Cepa da irradiação com o raio X

Autores

  • Mithitaka Soma
  • Angela Cristina Ito
  • Sérgio Carlos Barros Steves
  • Luciana Mari Tsutsui
  • Lilian Stranghetti Jorge
  • Maria Carolina Aoki
  • Milena Belotti Amorim

Resumo

Foram descritos os efeitos das radiações X em várias dosagens em Allium cepa, irradiados de 6 - 12 Gy. As análises foram feitas em 2000 células do ciclo celular e mostramos as principais anomálias visualizadas em microscópia óptica. Os efeitos da radiação X são muito drásticos, principalmente quando as dosagens de radiação vão aumentando, podendo-se visualizar micronúcleos, pontes de cromossomas edeteções.

Downloads

Não há dados estatísticos.

Referências

ABEND, M.; Frombeck, S. and Van Beuningen, D., 1999 Indications for a activeprocess underlyingspontaneous and radiation-induced micronucleation in L929 cells. Int. J. Radiat. Biol. Vol.75; Nº 12 - 1567-78.

ABEND, M.; Rhein, A.; Gilbertz. K. P.; Blakely, W. F. and Van Beuningem, D., 1995 - Correlation of micronucleus and apoptosisassays with reproductive cell death. Int. J. of Radiation Biol., 67, 315 - 26.

ABEND, M.; Rhein, A.; Gílbertz, K. P. and Van Beuningen, D., 1996ª - Evaluation of a modified micronucleus assay. Int. J. of Radiation Biol.,_69, 717,- 27.

AGER, D. D. and Dewey, W. C., 1990 - Calibration of pulsed field gel electrophoresis for measurement of DNA double strand breaks. Int. J. of Radiation Biol., 58, 242-259.

CORINTRYMAN, P. J. & Heddle, J. A., 1976 The production of micronuclei from chromosome aberrations inirradiated cultures ofhuman lymphocytes. Mutat. Res. 41: 321 - 32.

DI MAJO, V.; Coppola, M.; Rebessi, S.; Saran, A .; Pazzaglia, S.; Pariset, L. and Covelly, V., 1996 -The influence of sex on life shortening and tumor induction in CBA / Cne mice exposed to x - rays or fission neutrons. Radiation Research. Virginia: Carden Jennings Publishing. n. 146. p. 81 - 87.

ELLER, M. S.; Yaar, M. and Gilchrest, B. A., 1994 - DNA damage and melanogenesis. Nature. 372: 413 - 4.

EVANS,H.J.;Neary,G.J.andWillianson,F. S., 1959-The relative biological efficiency ofsingle doses ofthe fast neutrons and gamma-rays on Vicia faba roots and the effect of oxigen. Part II. Chromosome damage: the production of micronuclei. Int. J. Radit. Biol. 3: 216 - 29.

FENECH, M. & Morley, A. A. 1986 - Cytokinesis - block micronucleus method in human lymphocytes: effect of in vivo agling and low dose x-irradiation. Mutat. Res. .161: 193-8.

FUHRMANN, C.; Streffer, C.; Muller, W. U. and Bender, U., 1992 - Micronucleus assaypredictionand optimized by cytochalasin-B, induced benucleated tumor cells. Strahlenther onkol. 168: 6036 - 09.

GANTEMBERG, H. W.; Wuttke, K.; Streffer, C. and Muller, W. U., 1991 - Micronuclei in human lymphocytes irradiated in vitro or in vivo. Radiat. Res.. 128: 276 - 81.

HANDO, J. C.; Nath, J. and Tricker, J. D., 1994. Sex chromosomes, micronuclei and aging in women. Chromosoma. 103: 186 - 92.

HSU, T. C.; Johnston, D. A .; Cherry, L. M.; Ramkisson, D.; Schantz, P.; Jessup, J. M.; Winn, R. J.; Shirley, L. and Furlong, C.,1989 - Sensitivity to genotoseic effects of bleomycim in humans: possible relationship to environmental carcinogenesis. Int. J. cancer. 43: 403 - 09.

ILIAKIS, G., 1991 . The role ofDNA double strand breaks inionizing radiation induced killing of eucaryotic cells. Bioessays, 13, 641 - 48.

ISHII, K.; Hosoi, Y.; Yamada, S.; Ono, T. and Sakamoto, K., 1996 Decreased incidence ofThymic Lymphoma in AKR mice as a result of chronic, fractioneted low-dose total-body x-irradiation. Radiation Research. Virginia: carden Jennings Publishing 146. p. 582 - 585.

JIKKO, A.; Hiranuma, H.; lwamoto, M.; Kato, Y.; Okada, Y. and Fuchihata, H.,1996 Effects of x- irradiation on metabolism of proteoglycans. Radiation Research, Virginia: carden Jennings Publisbing. n. 146. p. 93 - 99.

KRAMER,J.; Schaich-Walch, G. & Nusse, M., 1990. DNA synthesis in radiation induced micronucleus studie by bromodeoxyridine (BrdUrd) labelling and anti BrdUrd antibodies. Mutagenesis. 5 (5) : 491 - 95.

KUMB,A ., 1994. Sunprotection factorp. 53. Nature. 372: 730 - 1.

LANE,D. P., 1993 - A death in the life in the ofp.53. Nature, 362: 786 - 7

LUOMAHAARA, S.; Lindholm, C.; Mustonen, R. and Salomaa, S., 1999. Distribution ofradiation­ induced exchange aberrations in human chromosomes 1, 2 and 4. Int. J. Radiat. Biol. Vol. 75, nº 12, 1551 - 56.

MAISIN, J. R.; Gerber, G. B.; Vanderkom, J. and Wambersie, A., 1996 - Survival and diseases in C57Bl mice exposed to x-rays or 3-1 Mev neutrons at na age of 7 or 21 days. Radiation Research. Virginia: carden Jennings Publishing, n. 146 p. 453 - 460.

MANTI, L.; Jamali, M.; Prise, M.; Michael, B. D. and Trott, K. R., 1997 - Genomic instability in Chinese Hamster cellsafterexposuretox-raysoralphaparticles of different mean linear energy transfer. Radiation Research. Virgínia: Carden Jennings Publishing. 147. p. 22 - 28.

MCFEE, A. F.; Cook, S. B. & Abbott, M. G., 1994. Negative dose-response relationship for radiation induced micronuclei in polychromatic reythrocytes of mice. Environ. Mol. Mutat. 23: 128 - 31.

MILL, A . J.; Wells, J.; Hall, S. C. and Buther, A., 1996 - Micronucleus induction in human lymphocytes. Comparative effects ofx-rays, alpha particles, beta particles and neutrons and implications for biological dosimetry. Radiation Research. Virgínia: Carden Jennings Publishing. n. 145 p. 575 - 585.

MIZUNO, T., 1997 - ln: A solução para o câncer através da alimentação. Editor - Teruki Matsumoto, Editora­ ção - Paulo's Comunicaçãoe Artes Gráficas Ltda.

MOLLS, M.; Beuningen, D. Van & Streffer, C., 1986 - The micronuclei assay in biological dosimetry. Bga­ Schriffen, 2: 248 - 52.

NATARAJAN, A. T., 1984 -Origin and significance of chromosome alterations. ln: Ed. Obe, G. Mutation in man, Springer-Verlag. Berlin. Heidelberg

NATARAJAN, A. T.; Darroudi, F.; Mullenders, L. H. F. and Meyers, M., 1986 - The nature and repair ofDNA lesions that lead to chromosome meal aberrations induced by ionizing radiationm. Mutation Research 160: 231 - 236.

NURSE, M.; Kramer, J. & Miller, B. M., 1992 - Factors influencing the DNA content of radiation-induced micronuclei. lnt. I. J. Radiat. Biol.62: 587 - 602.

RUSSEL, L. B., 1965 - Death and chromosome damage from irradiation of preimplantation stages. ln: Wolstenhome GEW, O'Connor, (Eds): Preimplantation stages of Pregnancy London: J & A Churchil Ltda. Pp. 217.

STREFFER, C., 1993 - Is the micronucleus assay predictive for cellularradiosensitivity.Br. J. Radio1. 66 (Suppl. 24): 70 - 3.

Downloads

Publicado

2000-12-31

Como Citar

Soma, M., Ito, A. C., Steves, S. C. B., Tsutsui, L. M., Jorge, L. S., Aoki, M. C., & Amorim, M. B. (2000). Os efeitos em Allium Cepa da irradiação com o raio X. Bioikos – Título não-Corrente, 14(2). Recuperado de https://seer.sis.puc-campinas.edu.br/bioikos/article/view/931

Edição

Seção

Artigos